松樹(shù)體細胞胚胎發(fā)生與超低溫凍存技術(shù)（一本超實(shí)用的體細胞胚胎發(fā)生與超低溫凍存技術(shù)圖書(shū)?。?/title> <link rel="stylesheet" type="text/css" href="/css/swiper.min.css" /> <link rel="stylesheet" type="text/css" href="/css/base.css" /> <link rel="stylesheet" type="text/css" href="/css/animate.css"> <link rel="stylesheet" type="text/css" href="/css/page.css" /> <link rel="stylesheet" type="text/css" href="/css/media.css" /> <meta http-equiv="Cache-Control" content="no-transform" /> <meta http-equiv="Cache-Control" content="no-siteapp" /> </head> <body> <a href="http://www.shouder.cn/">2020最新无码片中文字幕|国产欧美日产高清欧美一区二区|2021国产精品手机在线|国产乱伦|免费在线观看。黄片_亚洲成av人精品自偷拍_国产免费午夜a无码v视频_夜色资源站WWW国产</a> <div id="pl_css_ganrao" style="display: none;"><var id="7z7wp"><tr id="7z7wp"><listing id="7z7wp"><sub id="7z7wp"></sub></listing></tr></var><acronym id="7z7wp"></acronym><menuitem id="7z7wp"><wbr id="7z7wp"><video id="7z7wp"></video></wbr></menuitem><font id="7z7wp"></font><nobr id="7z7wp"><center id="7z7wp"><thead 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書(shū) 號： 978-7-5359-6934-7<br /> 主編：郭文冰，趙奮成，王為民<br /> 開(kāi) 本： 16開(kāi)<br /> 頁(yè) 數： 96頁(yè)<br /> 字數： 150千字<br /> 出版時(shí)間：2018年04月24日<br /> 定價(jià)： 68元 </div> </div> </div> </div>   <div id="27evmjt" class="box-2 mt wow fadeInUp50"> <div id="tpdb7pe" class="box-container"> <div id="hkb7vrd" class="tab-box wow fadeInUp50"> <a href="javascript:;">主編簡(jiǎn)介</a> <a href="javascript:;">內容簡(jiǎn)介</a> <a href="javascript:;">目錄預覽</a> </div> <div id="bp7qwpv" class="detail-box wow fadeInUp50"> <div id="tbp9jnb" class="item detail-1"> <ul> <li> <div id="ykf9j7i" class="desc"> 郭文冰,廣東省林業(yè)科學(xué)研究院副研究員，廣東省森林培育與保護利用重點(diǎn)實(shí)驗室副主任。曾在美國佐治亞大學(xué)開(kāi)展博士后研究，主要從事松樹(shù)遺傳改良與森林培育，先后主持國家自然科學(xué)基金、廣東省自然科學(xué)基金、省級科技計劃等7個(gè)項目。<br /> </div> </li> </ul> </div> <div id="722fttt" class="item detail-2"> <div id="27pj27q" class="content"> 本書(shū)的內容由四大部分組成。著(zhù)重介紹了松樹(shù)體細胞胚胎發(fā)生技術(shù)的主要操作環(huán)節，從體細胞胚胎室的建設、外植體采集，到體細胞胚胎誘導、增殖、成熟、萌發(fā)與植株再生等。詳細介紹超低溫凍存的生物學(xué)原理、技術(shù)原理及主要研究進(jìn)展，并提供了針葉樹(shù)種胚性細胞凍存與解凍的具體操作步驟。 <p> 本書(shū)內容重點(diǎn)突出，理論深度適宜，實(shí)用性強，可作為科研院所、高校掌握植物體細胞胚胎發(fā)生與超低溫凍存相關(guān)原理的參考書(shū)，也可作為科技人員開(kāi)展相關(guān)研究的技術(shù)手冊。</p> </div> </div> <div id="zpo7tx9" class="item detail-3"> <div id="crjp77b" class="content"> 1　植物體細胞胚胎發(fā)生概況與原理............... 1<br />1.1　合子胚發(fā)生過(guò)程 .................................2<br />1.1.1　被子植物合子胚發(fā)生 .......................3<br />1.1.2　裸子植物合子胚發(fā)生 .........................6<br />1.2　體細胞胚胎發(fā)生過(guò)程........................6<br />1.2.1　胚性培養物的誘導與增殖.......................11<br />1.2.2　體細胞胚的成熟與發(fā)育 .........................12<br />1.3　合子胚發(fā)生與體細胞胚胎發(fā)生的異同................ 14<br />1.3.1　形態(tài)與組織結構的異同點(diǎn)...........................15<br />1.3.2　生化方面的異同點(diǎn).....16<br />1.3.3　合子胚與體細胞胚胎發(fā)生的差異機制研究..................18<br />1.4　體細胞胚胎發(fā)生的調控研究 ...... 19<br />1.4.1　體細胞胚胎發(fā)生的動(dòng)態(tài)追蹤..........................19<br />1.4.2　影響細胞分化的信號分子研究 ...................... 20<br />1.5　體細胞胚胎發(fā)生研究歷程........... 21<br />1.5.1　植物體細胞胚胎發(fā)生研究史上的里程碑 .................21<br />1.5.2　松樹(shù)體細胞胚胎發(fā)生研究歷程 ....................... 24<br />2　松樹(shù)體細胞胚胎發(fā)生技術(shù) ........................ 29<br />2.1　松樹(shù)體細胞胚胎發(fā)生研究的前期準備..................... 30<br />2.1.1　體細胞胚胎室的建設 ........................... 30<br />2.1.2　試劑耗材與培養基配制 ................................ 36<br />2.2　松樹(shù)體細胞胚胎發(fā)生外植體選擇....................... 38<br />2.2.1　材料的來(lái)源 ...........38<br />2.2.2　不同合子胚發(fā)育階段外植體..........................39<br />2.3　松樹(shù)體細胞胚胎發(fā)生的誘導 ...... 42<br />2.3.1　誘導培養基配方.......42<br />2.3.2　胚性愈傷組織的誘導 .................................44<br />2.3.3　胚性愈傷的鑒別......45<br />2.4　松樹(shù)體細胞胚胎維持與增殖 ...... 45<br />2.4.1　胚性愈傷的固態(tài)增殖 .................................45<br />2.4.2　胚性愈傷的液態(tài)增殖 ............................... 46<br />2.5　松樹(shù)體細胞胚胎發(fā)育與植株再生......................... 47<br />2.5.1　松樹(shù)體細胞胚胎的成熟 ...........................47<br />2.5.2　松樹(shù)體細胞胚胎萌發(fā)與植株再生 ........................52<br />2.5.3　松樹(shù)體細胞胚胎遺傳穩定性分析 ........................55<br />3　體細胞胚胎的超低溫凍存技術(shù)................. 57<br />3.1　體細胞胚胎超低溫保存的原理與研究進(jìn)展 ................. 58<br />3.1.1　體細胞胚胎超低溫保存的概念 ..........................58<br />3.1.2　超低溫保存的基本原理 ................................ 60<br />3.1.3　超低溫保存機制的研究 .................................65<br />3.2　針葉樹(shù)種胚性細胞常用的兩種超低溫保存技術(shù)............... 68<br />3.2.1　胚性細胞程序降溫方法步驟.............................69<br />3.2.2　胚性細胞玻璃化方法步驟................................71<br />3.2.3　保存材料的活性檢測 ...................................74<br />4　體細胞胚胎發(fā)生技術(shù)的應用與前景......... 77<br />4.1　體細胞胚胎發(fā)生技術(shù)在遺傳改良上的應用 .................. 78<br />4.1.1　體細胞胚胎無(wú)性系應用于遺傳測定.......................78<br />4.1.2　雜種后代的胚拯救....................................79<br />4.2　體細胞胚胎發(fā)生技術(shù)在人工培育上的應用 .................. 79<br />4.2.1　基于體細胞胚胎的無(wú)性系林業(yè) .......................79<br />4.2.2　體細胞胚胎苗木與人工種子的利用........................82<br />4.3　體細胞胚胎發(fā)生介導的遺傳轉化......................... 83<br />4.3.1　松樹(shù)遺傳轉化的方法 ...........................83<br />4.3.2　基于體細胞胚胎發(fā)生的遺傳轉化體系..................85<br />4.4　體細胞胚胎發(fā)生與藥用蛋白質(zhì)的生產(chǎn)...................... 86<br />4.4.1　藥用轉基因植物細胞的利用............86<br />4.4.2　針葉樹(shù)種藥用蛋白的生產(chǎn)..................87 </div> </div> </div> </div> </div>   <div id="729k9o7" class="box-3 index-box mt"> <div id="orz7dtr" class="box-container"> <div id="772rfqb" class="index-title-box txt-center wow fadeInUp50"> <p>Related books</p> <h3>相關(guān)圖書(shū)</h3> </div> <div id="okbw77u" class="swiper-box wow fadeInUp50"> <div id="rrlkap2" class="swiper-container recommend-swiper"> <div id="tpvbv92" class="swiper-wrapper"> <div id="xxrxnrg" class="swiper-slide"> <a href="/Latest/book_5325.html"> <div id="v7nt7d9" class="pic"> <img 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